More lab lamenting

May 31 2009 Published by under Uncategorized

So we think we nailed down which of our plastics were giving us some of the background. But we still can't get any signal. So, we did some other controls and realized that the new lot of one of our reagents is about 1000X less sensitive than the old lot. A conversation with tech support later tells us that this is the only lot currently available. Hmmm. They even sent us a whole new batch (of same lot, though, since there aren't any others) and it is still only able to detect 1000X higher concentrations of the stuff we're looking for compared to our previous lot (which is all gone by now).

So, this sucks. We're stuck. This is a reagent I had already optimized as the most sensitive for what we were trying to do. We can try one other version of it, of which the company is sending a sample, but based on what I have done before I have a feeling it won't work as well. And this is all we need to publish our first paper. 🙁

4 responses so far

  • ScientistMother says:

    that sucks ass. i'm sorry

  • Comrade PhysioProf says:

    Undocumented 1000-fold difference in sensitivity from batch to batch!?!?!? What the fuck??

  • Arlenna says:

    Yah. This is a new lot for them, and we're the first customers to complain about it, so maybe something went wrong that nobody else has noticed yet?

  • Mad Hatter says:

    I guess it would be too much to ask for for a company to QC their products before selling it? Sheesh! I'm telling ya...it's the paper gremlins. Anytime you try to publish a paper, something that absolutely shouldn't go wrong does. I need to do one additional experiment for a manuscript resubmission and suddenly, I'm incapable of performing PCR successfully....

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