Purty, but missing the green.

May 25 2009 Published by under Uncategorized

7 responses so far

  • ScientistMother says:

    Ooh lala! That is purty. I love how beautiful dapi always looks!!

  • Comrade PhysioProf says:

    In some cells the red shit looks like something in the ER, but in others it looks like maybe actin in the filopodia.

  • Arlenna says:

    Either one of those things could be pretty cool. I still need the green to know how significant it could be, though. I also need all those organelle stains (damn those things have a lot of background in what I've tried!).

  • Toaster Sunshine says:

    Have you tried using a wicking clamp and capillary slides? Gives me wonderful resolution, and I only have to use ~75ul per slide of any given stain.

  • Arlenna says:

    These are live cells, so I can't do that. Would love to be able to! 😛 These experiments don't work in fixed cells so I have no choice.

  • Toaster Sunshine says:

    What about frozen sections? That, at least, doesn't alter epitopes.Of course, I have no idea what you're looking for so I could well be talking out of my ass.

  • Arlenna says:

    heh. Nope, still a no-go. The cells have to be metabolically functional for our research to be interesting. 🙂 We're watching dynamics of stuff in living individual cells.

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